Santa Cruz Biotechnology offers conjugated and unconjugated monoclonal primary antibodies directed against most steroid receptors. Steroid receptors are nuclear receptors typically found in the cytoplasm. Upon steroid ligand binding, the steroid receptor translocates into the nucleus where it acts as a transcription factor and directs gene expression. Steroid receptor activity has a profound influence on development, metabolism, and organism homeostasis.
Most of our popular antibodies are also available as ImmunoCruz ® Conjugates , including AC , HRP , PE , FITC , Alexa Fluor ® 488 , 546 , 594 , 64 7, 680 , and 790 conjugates for direct Western Blotting and Staining applications, eliminating the need for a secondary antibody. Additional conjugates are also available for many antibodies, including Biotin, TRITC, PE-Cy5, PE-Cy7, APC, APC-Cy7, Alexa Fluor ® 405, PerCP and PerCP-.
This paper presents the development, optimization and validation of a methodology to determine nine key steroid hormones (viz. pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone, dihydrotestosterone, estrone, 17α-estradiol and 17β-estradiol) expressed in the steroidogenesis in biological fluids. The analytical method allows for the determination of steroid hormones in blood plasma and serum down to - ng/mL for estrogens, - ng/mL for androgens and - ng/mL for progestagens. These limits of detection were obtainable using a two-step solid-phase clean-up for fractionation and elimination of interfering lipids (fatty acids, phospholipids, glycerides and sterols) from the steroid hormones. The accuracy of the method was 50-112% in the range to ng/mL.